2001•Xibei zhiwu xuebaoRequires access

RFLP analysis of mtDNA between two types of cytoplasmic male sterility and their associated lines in rice

Fang Yao

Abstract

BT and WA type rice cytoplasmic male sterility (cms) lines were compared with their maintainer,restorer and F 1 hybrid by using RFLP analysis.The results showed:(1) polymorphism were found between BT and WA type cms lines;(2) difference could be detected between cms line and its maintainer,so we inferred that mtDNA may be associated with cms in rices;(3) RFLP polymorphic patterns were identified between cms line and its F 1 hybrid by using atp9,Frag36 and Frag9 as probes,which revealed that nuclear background has an influence on the existence of mitochondrial DNA;(4) relationship between organization of mitochondiral and cytoplasmic male sterility was discussed.

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Key concepts: Cytoplasmic male sterility, Software maintainer, Restriction fragment length polymorphism, Sterility, Mitochondrial DNA, Biology, Genetics, Polymorphism (computer science)