Optimization of the backward extraction of protein from peanut by reverse micelles

The backward extraction of peanut protein from forward extraction solution was studied.The reverse micellar systems were formed by sulphosuccinic acid bis(2-ethylhexyl) ester sodium salt(AOT),isooctane and KCl buffer solution.The effects of extraction time,extraction temperature,pH and KCl concentration on the backward extraction ratio were tested.On the basis of single-factor experiments,the optimum technical condition was obtained by orthogonal experiments.The results showed that the highest backward extraction ratio of protein were reached at the condition of extraction time 50 min,extraction temperature 40℃,pH 9.0 and KCl concentration 1.0 mol/L.Under this optimum condition,the backward extraction ratio of protein reached 86.49%.