2014Cereals & OilsRequires access

Optimization of backward extraction of peanut protein by enzyme-containing reverse micelles

Yang Chen-xia

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Abstract

This paper studied the backward extraction of peanut protein by enzyme–containing reverse micelles. And the effects of the pH of buffer solution,KCl concentration,the temperature of extraction and oscillation time were investigated. The study designed by custom design function of JMP software and establish the mathematical model of peanut protein extraction. The optimum conditions of backward extraction conditions of peanut protein in enzyme–containing reverse micelles were the pH 8.06,KCl concentration 1.53 mol/L,temperature 40 ℃ and oscillation time 120 min. And the backward extraction rate was(78.43±0.87)%.

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What this paper is about

This paper studied the backward extraction of peanut protein by enzyme–containing reverse micelles. And the effects of the pH of buffer solution,KCl concentration,the temperature of extraction and oscillation time were investigated. The study designed by custom design function of JMP software and establish the mathematical model of peanut protein extraction. The optimum conditions of backward extraction conditions of peanut protein in enzyme–containing reverse micelles were the pH 8.06,KCl concentration 1.53 mol/L,temperature 40 ℃ and oscillation time 120 min. And the backward extraction rate was(78.43±0.87)%.

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Available abstract

This paper studied the backward extraction of peanut protein by enzyme–containing reverse micelles. And the effects of the pH of buffer solution,KCl concentration,the temperature of extraction and oscillation time were investigated. The study designed by custom design function of JMP software and establish the mathematical model of peanut protein extraction. The optimum conditions of backward extraction conditions of peanut protein in enzyme–containing reverse micelles were the pH 8.06,KCl concentration 1.53 mol/L,temperature 40 ℃ and oscillation time 120 min. And the backward extraction rate was(78.43±0.87)%.

Key concepts: Extraction (chemistry), Micelle, Chemistry, Enzyme, Chromatography, Protein purification, Biochemistry, Aqueous solution

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