Macroporous Resin Adsorption for Purification of Crude Flavonoids from Bamboo Leaves

To screen an optimal adsorbent for purification of flavonoids from bamboo leaves,7 macroporous resins were compared for difference in adsorption and desorption performance.AB-8 resin was chosen due to the merits of large adsorption capacity,easy desorption and high resolution.Besides,the optimal adsorption/desorption process for purification of bamboo leaf flavonoids using AB-8 resin was investigated.Results showed that the optimal pH and flow rate of sample solution were 5.0 and 1 mL/min,respectively and the optimal desorption was achieved by using 4 BV of 60% aqueous ethanol at a flow rate of 1.5 mL/min.Under such conditions,the purity of bamboo leaf flavonoids obtained was up to 54.16%.