A study on the purification of the flavonoids from aloe leaf powder with macroporous resin.
Yang WuYing, Xin-Chen Shang-Guan, WU Lei-yan, Yong Yang, Hongmin Li, Jiang Xing
Abstract
Yang WuYing, Xin-Chen Shang-Guan, WU Lei-yan, Yong Yang, Hongmin Li, Jiang Xing
Abstract
The purification of the flavonoids from aloe leaf powder with macroporous resin was studied.The static adsorption rate and desorption rate of five different macroporous resins were surveyed under the same conditions to select the best one.The optimum purificated technic was confirmed by studying the static and dynamic absorption and desorption of flavonoids with the best resin under different conditions.The result indica-ted that the optimum macroporous resin was H1020,and its static adsorptive ability was the strongest at room temperature and pH=3,the most effective desorption could be obtained with 80% volume fraction alcohol.The best dynamic absorption was obtained at room temperature and crude flavonoids solution at 12 mg/mL,the most effective desorption was also obtained with 80% volume fraction alcohol at 2.0 mL/min desorption rate.
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The purification of the flavonoids from aloe leaf powder with macroporous resin was studied.The static adsorption rate and desorption rate of five different macroporous resins were surveyed under the same conditions to select the best one.The optimum purificated technic was confirmed by studying the static and dynamic absorption and desorption of flavonoids with the best resin under different conditions.The result indica-ted that the optimum macroporous resin was H1020,and its static adsorptive ability was the strongest at room temperature and pH=3,the most effective desorption could be obtained with 80% volume fraction alcohol.The best dynamic absorption was obtained at room temperature and crude flavonoids solution at 12 mg/mL,the most effective desorption was also obtained with 80% volume fraction alcohol at 2.0 mL/min desorption rate.
Key concepts: Desorption, Adsorption, Chromatography, Chemistry, Volume fraction, Fraction (chemistry), Volume (thermodynamics), Mass fraction