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Determination of paeoniflorin in Fuyankang Pills by HPLC

Wang Xiao-gen

Abstract

Objective:To establish a method for the determination of paeoniflorin in Fuyankang Pills by HPLC.Methods:The HPLC method was performed on a Diaminsil C18 ODS column(5 μm,4.6×200 mm),the composition of mobile phase was acetonitrile-0.05% potassium phosphate monobasic solution(14:86),the wavelength was 230nm.Results:The linear range of paeoniflorin was 0.304～1.216 μg(r=0.9997).The average recovery rate of paeoniflorin was 99.5 % with RSD of 1.05%.Conclusion:This method is accurate,reliable and reproducible.It can be used for assaying of paeoniflorin in Fuyankang Pills.

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Key concepts: Paeoniflorin, Monobasic acid, Medicine, Chromatography, High-performance liquid chromatography, Potassium phosphate, Chemistry, Polymer chemistry