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Determination of Paeoniflorin in Shedan Zhuifeng Pills by HPLC

Gao Mao-guo

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Abstract

Objective To establish a method of measuring paeoniflorin in Shedan Zhuifeng pills by HPLC. Methods 18 alkyl silane bonded silica was used as the chromatorgram column for filler, with acetonitrile-0.1% phosphoric acid (10 ∶ 90) as the mobile phase. Detection wave length was at 230 nm. Theoretical plate number calculated by paeoniflorin peak should not be less than 3500. Results When Paeoniflorin reference substance was between 4.036~40.360 μg/ml, its peak area would be in good linear relationship. The recovery rate was 99.51%, RSD was 0.2%. Conclusion This method is specific, sensitive, accurate, and can be used for the quality control of Shedan Zhuifeng pills.

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What this paper is about

Objective To establish a method of measuring paeoniflorin in Shedan Zhuifeng pills by HPLC. Methods 18 alkyl silane bonded silica was used as the chromatorgram column for filler, with acetonitrile-0.1% phosphoric acid (10 ∶ 90) as the mobile phase. Detection wave length was at 230 nm. Theoretical plate number calculated by paeoniflorin peak should not be less than 3500. Results When Paeoniflorin reference substance was between 4.036~40.360 μg/ml, its peak area would be in good linear relationship. The recovery rate was 99.51%, RSD was 0.2%. Conclusion This method is specific, sensitive, accurate, and can be used for the quality control of Shedan Zhuifeng pills.

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Available abstract

Objective To establish a method of measuring paeoniflorin in Shedan Zhuifeng pills by HPLC. Methods 18 alkyl silane bonded silica was used as the chromatorgram column for filler, with acetonitrile-0.1% phosphoric acid (10 ∶ 90) as the mobile phase. Detection wave length was at 230 nm. Theoretical plate number calculated by paeoniflorin peak should not be less than 3500. Results When Paeoniflorin reference substance was between 4.036~40.360 μg/ml, its peak area would be in good linear relationship. The recovery rate was 99.51%, RSD was 0.2%. Conclusion This method is specific, sensitive, accurate, and can be used for the quality control of Shedan Zhuifeng pills.

Key concepts: Paeoniflorin, Phosphoric acid, Chromatography, High-performance liquid chromatography, Chemistry, Acetonitrile, Organic chemistry

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