RP-HPLC Analysis of d1-Tetrahydropalmatine and Protopine in Corydalis yanhusuo Produced from Shaanxi

[Objective]The research aimed to establish a method for determination of d1-Tetrahydropalmatine and protopine in Corydalis yanhusuo.[Method]HPLC separation was carried on a column of ODS(4.6 mm×150 mm,5 μm),with the mobile phase of 0.1% phosphate buffer-methanol(65∶35) at the flow rate of 1.0 ml/min,and the detection wavelength was set at 280 nm.[Result]The average content of d1-Tetrahydropalmatine and protopine in Corydalis yanhusuo produced from was 0.977 and 0.651 mg/g respectively.Their average recovery were 98.02%(RSD=1.87%) and 98.63%(RSD=1.43%).[Conclusion]Compared with other literatures,the content of two active components in Corydalis yanhusuo W.T.Wang from Shaanxi,the content of d1-Tetrahydropalmatine and protopine were relative higher,which could meet the content requirements of China Pharmacopeia(2005).