Determinations of Active Compositions of Corydalis yanhusuo from Different Areas

Objective To study the content of active compositions of Corydalis yanhusuo in different area, provide some academic basis for quality criterion of Corydalis yanhusuo. Methods Studying and optimizing the methods of determination of HPLC of Corydalis yanhusuo, to establish the method of determination with HPLC, and using this method to determine the content of protopine, berberine and tetrahydropalmatine for Corydalis yanhusuo, using the method of potentiometric titration to determine the content of total alkaloids of Corydalis yanhusuo. Results The content of protopine was 0.2994 ~ 1.2419 mg/g; the content of berberine was 0.0839 ~ 0.1524 mg/g; the content of Tetrahydropalmatine was 0.4492 ~ 1.6504 mg/g; The content of total alkaloid was 4.03 ~ 6.48 mg/g. Conclusion The content of active compositions were significantly different in different areas of Corydalis yanhusuo.