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2005•Journal of Shaanxi Normal UniversityRequires access

RP-HPLC simultaneous determination of tetrahydropalmatine and protopine in Rhizoma Corydalis

Yi Wang

Open publisher page 2 citations

Abstract

A reserved-phase HPLC method is established for simultaneous determination of the content of tetrahydropalmatine and protopine in Rhizoma Corydalis from different habitats. The samples are separated on a Phenomenex Luna C_(18) column with mobile phase methanol-phosphate buffer (pH 768, 65∶35) and detected at wavelength of 281 nm. The average recoveries respectively for tetrahydropalmatine and protopine are 10167% and 9813%, RSD are 144% and 137%. The contents of the two alkaloid in Rhizoma Corydalis from different habitats show obvious difference. The method is simple, accurate, quick and suitable to determine content and evaluate quality for the drugs.

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What this paper is about

A reserved-phase HPLC method is established for simultaneous determination of the content of tetrahydropalmatine and protopine in Rhizoma Corydalis from different habitats. The samples are separated on a Phenomenex Luna C_(18) column with mobile phase methanol-phosphate buffer (pH 768, 65∶35) and detected at wavelength of 281 nm. The average recoveries respectively for tetrahydropalmatine and protopine are 10167% and 9813%, RSD are 144% and 137%. The contents of the two alkaloid in Rhizoma Corydalis from different habitats show obvious difference. The method is simple, accurate, quick and suitable to determine content and evaluate quality for the drugs.

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Available abstract

A reserved-phase HPLC method is established for simultaneous determination of the content of tetrahydropalmatine and protopine in Rhizoma Corydalis from different habitats. The samples are separated on a Phenomenex Luna C_(18) column with mobile phase methanol-phosphate buffer (pH 768, 65∶35) and detected at wavelength of 281 nm. The average recoveries respectively for tetrahydropalmatine and protopine are 10167% and 9813%, RSD are 144% and 137%. The contents of the two alkaloid in Rhizoma Corydalis from different habitats show obvious difference. The method is simple, accurate, quick and suitable to determine content and evaluate quality for the drugs.

Key concepts: Tetrahydropalmatine, Protopine, Corydalis, Chemistry, Chromatography, High-performance liquid chromatography, Alkaloid, Stereochemistry

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RP-HPLC simultaneous determination of tetrahydropalmatine and protopine in Rhizoma Corydalis — Research Paper | ScholarLens