The Effects of Calcitonin Gene Related Peptide and Nerve Growth Factor on the Expression of FasmRNA in the Parietal lobe and Hippocampus during Focal Cerebral Ischemia/ Reperfusion in Rats

Objective To investigate the effects of exogenous calcitonin gene related peptide(CGRP) and nerve growth factor(NGF) on the expression of FasmRNA in rat parietal lobe and hippocampus with focal cerebral ischemia/reperfusion(I/R)and to explore the effect and mechanism of CGRP and NGF on ischemia brain tissue.Methods Using middle cerebral artery occlusion(MCAO) to make focal cerebral ischemia model,the expression of FasmRNA in parietal lobe and hippocampus was detected with in situ hybridization method and analyzed by microimage analysis system.Results FasmRNA positive cells were not found in parietal lobe and hippocampus of sham group,but found obviously in focal cerebral I/R group(6h,12h,24h,48h and 72h after I/R).The average optical density(OD) value of FasmRNA positive product in CGRP or NGF groups was lower than that in I/R group(P0.01),and the average OD value of FasmRNA positive product was lower in CGRP NGF group than in CGRP or NGF group(P0.05).Conclusion Exogenous CGRP and NGF through carotid artery injection can downregulate the expression of FasmRNA in ischemic neuron of parietal lobe and hippocampus,which may be one of the mechanisms for the protective effect of the ischemic neuron by CGRP and NGF.