The effect of CGRP and NGF on the expression of PKC during regional cerebral ischemia and reperfusion in rats

Objective To investigate the expression of protein kinase C (PKC) in hippocampus and parietal cortex after whole cerebral ischemia and reperfusion in rats to explore the protective effects and mechanism of calcitonin gene-related peptide (CGRP) and nerve growth factor (NGF) on ischemic brain tissue. Methods An novel model of whole cerebral ischemia and reperfusion induced by carotid artery negative pressure was extablished in rats, the expression of PKC was detected with SABC immunohistochemical method and analyzed by microimage system. Results The average gray values of PKC positive product in hippocampus CA 1 and parietal cortex were lower in cerebral ischemia/reperfusion group than in sham control group (P0.05). The average gray values of PKC positive product in CGRP or NGF group were higher compared with cerebral ischemia/reperfusion group (P0.01). The average gray values of PKC was higher in NGFCGRP group than in CGRP or NGF group respectively (P0.01). Conclusion The results indicate that CGRP and NGF participate in the regulation of expression of PKC in ischemic neurons,and they may cooperate with each other.