Expression of Bmi-1 and p16 in cervical carcinoma carcinogenesis progression and its significance

Objective To study the expressions of Bmi-1 and p16 in tissues of normal,cervical intraepithelial neoplasia(CINⅠ,CINⅡ,ClNⅢ)and cervical cancer.Methods The expressions of Bmi-1 and p16 in tissues of 10 cases of normal cervical epithlium,48 cases of CINⅠ-Ⅲ and 32 cases cervical cancer were detected by immunohistochemical(S-P) method.The expressions of the two proteins in 18 freshly——taken cervical carcinoma and adjacent cervical tissue specimens were detected by Western Blot.Results The positive rates of Bmi-1 expression showed a gradual increase in cervical cancer progression,but p16 expression showed a gradual decrease.No obvious correlation was found in the expression of the two proteins with regard to the age of the patiens or pelvic lymph node metastasis.However,the expression of Bmi-1 and p16 had a correlation with pathological stage(P=0.002,0.003).The levels of Bmi-1 in cervical cancer were higher than those in the paraneoplastic tissue(P=0.002,0.003),but the levels of p16 were lower.Conclusion To detect the expression of the levels of the two proteins could suggest,to some extent,the potential transformation of cervical intraepithelial neoplasia to cervical cancer.