EXPERIMENTAL STUDY ON PROTECTIVE EFFECTS OF ANISODAMINE ON ISCHEMIA-REPERFUSION INJURY IN RAT LIVER

Objective To study the protective effects of anisodamine on liver ischemia reperfusion injury in rats. Methods One hundred and sixty male Wistar rats were randomly divided into the normal control ( n= 10), ischemia reperfusion ( n =50), normal saline ( n =50) and anisodamine ( n =50, 2.0 mg/kg) groups. The animals were killed 1, 3, 6, 12, 24 hours after ischemia induced for 60 minutes and followed by reperfusion. Plasma endothelin 1 (ET 1), hyaluronic acid (HA), glutamic pyruvic transaminase enzyme (ALT) were measured, and the hepatic histopathologic alterations were also observed. Results The plasma ET 1, HA and ALT concentrations were markedly increased after liver ischemia reperfusion.The hepatic congestion was significantly obvious. An intravenous injection of anisodamine before ischemia reperfusion could decrease the plasma HA and ALT concentrations and relieve the hepatic congestion. Conclusion Anisodamine can improve hepatic microcirculatory disturbances after reperfusion and have hepatoprotictive effects on rat liver ischemia reperfusion injury.