Experimental study of injure and its mechanism in the ischemia and reperfusion period in rabbit liver

Objective To study the injury a nd its mechanism in the ischemia and reperfusion period in rabbit liver. Methods Twenty four rabbits were randomly divided into the ischemia an d reperfusion group and normal control group.Hepatic tissue oxygeon pressure(P ti O 2) was measured before,during,and after the ischemic period in the left lobe.Plasma glutamic pyruvic transaminase enzyme(ALT) was also measured.The hi stopathologic alteration of the liver was observed.Results ALT and P ti O 2 value in the ischemia and reperfusion group at liver ischemia reperfusion different periods remained significantly higher than that in the c ontrol group(P0.05).Meanwhile,the histopathologic injury of li ver were significantly remarkable in the ischemia reperfusion group.Con clusion The liver ischemia reperfusion injury may be induced by Pringl e maneuver.The mechanism is associated with cell anoxia,the disorder of microcir culation and injury of mitochodria of hepatic cells during ischemia.