Modulating Effect of Celecoxib to Proliferation and Molecular Mechanism in MGC803 of Gastric Carcinoma Cell Line

Objective :To study celecoxib in vitro human gastric cancer cell growth and proliferation of MGC803 and its anti-tumor molecular mechanisms.Methods Cultured human gastric cancer MGC803,MTT method analyses celecoxib at the same concentration,at different times for the gastric cancer cell proliferation and calculate IC50 value;RT-PCR method used to evaluate the expression of COX-2,MMP-9 mRNA.Results MTT assay showed: the concentration of the same intervention celecoxib to inhibit the proliferation of human gastric cancer cells,its 24h,48h,72h IC50 of respectively: 98.67±11.755μmol/L、67.506±6.646μmol/L、57.662±15.809μmol/L;The RT-PCR results show: human gastric cancer cells MGC803 normal control group and celecoxib in the intervention group,respectively,the COX-2mRNA gray value for: 0.918±0.031,0.301±0.002(t=16.037,P=0.000);MMP-9 mRNA gray values were: 0.808±0.021,0.101±0.002(t=19.037,P=0.000);MMP-9 mRNA were 0.928±0.041,0.360± 0.012(t=10.487,P=0.003);Conclusions celecoxib inhibition of gastric cancer cell proliferation,celecoxib antitumor mechanisms may by inhibiting the expression of COX-2 and MMP-9 mRNA in implementation.