Effect of celecoxib on proliferation of BGC-823 in gastric carcinoma cell line and the molecular mechanism

Objective To study the effect of celecoxibon on human gastric cancer cell growth and proliferation of BGC-823 in vitro and its anti-tumor molecular mechanisms.Methods Human gastric cancer BGC-823 were cultured.At the same concentration,MTT method were used to analyse the effect of celecoxib on the gastric cancer cell proliferation at different times and calculate IC50 value;RT-PCR method was used to evaluate the expression of COX-2,MMP-9 mRNA.Results MTT assay showed:the same intervent concentration of celecoxib to inhibit the proliferation of human gastric cancer cells,its 24 h,48 h,72 h IC50 was respectively:158.98 μmol/L,75.45 μmol/L,45.08 μmol/L;The RT-PCR results showed that in human gastric cancer cells BxPC-3 normal control group and celecoxib in the intervention group,the COX-2 mRNA gray value were 0.873±0.026 and 0.115±0.008,P0.05;MMP-9 mRNA gray values were 0.890±0.011 and 0.209±0.012,P0.05.Conclusions Celecoxib could inhabit the proliferation of gastric cancer cell,inhabiting the expression of Cox-2 and MMP-9 mRNA may be its possible mechanism.