Modulating effect of celecoxib to proliferation and the molecular mechanism in Caco-2 of colon carcinoma cell line

Objective To study celecoxib in vitro human colon cancer cell growth and proliferation of Caco-2and its anti-tumor molecular mechanisms.Methods Cultured human colon cancer Caco-2,MTT method analyses celecoxib at the same concentration,at different times for the colon cancer cell proliferation and calculate IC50 value.RT-PCR method used to evaluate the expression of COX-2,Survivin mRNA.Results MTT assay showed:the concentration of the same intervention celecoxib to inhibit the proliferation of human colon cancer cells,its 24 h,48 h,72 h IC50 of respectively:(99.519 ± 10.355) μmol/L,(71.546 ± 6.446) μmol/L,(59.622 ± 15.999) μmol/L.The RT-PCR results show:human colon canoer cells Caco-2 normal control group and celecoxib in the intervention group,respectively,the COX-2 mRNA gray value for:0.873 ±0.026,0.115 ±0.008,P ＜ 0.05 ; SURVIVIN mRNA gray values were:0.808 ± 0.021,0.101 ± 0.002 (t =19.037,P =0.000) ; Survivin mRNA were0.798±0.031,0.190±0.002(t=18.987,P=0.002).Conclusions Celecoxib inhibition of colon cancer cell proliferation,celecoxib antitumor mechanisms may by inhibiting the expression of COX-2 and Survivin mRNA in implementation. Key words: Colonic neoplasms;  Celecoxib;  Hyperplasia;  COX-2;  Survivin