Study on Tissue Culture and Propagation System of DaSailaikete Strawberry

Adopted Dasailaikete Strawberry's stolon stem apex as the incept materials,in vitro cultivate and differentiate regeneration plants.Through further testing,filtrated appropriate medium for bud differentiation,proliferation and rooting medium,system established higher frequency of a new strawberry variety on tissue culture.The results showed :the best medium for explants survival rate was 1/2MS.Used MS+6-BA 0.5 mg/L+NAA 0.01 mg/L medium to induce adventitious buds,In MS+6-BA 0.6 mg/L+IBA 0.06 mg/L medium propagation coefficient higher up to 9.73 times and had the most effective plants.Rooting with 1/2MS+IBA 0.5 mg/L+NAA 0.02 mg/L+AC 0.15% medium,the rate was as high as 100.00%.Best practice was humus soil matrix Miller∶sand 3∶1,the survival rate got more than 90%,transplant field after two weeks,the survival rate was 98% and growing better.