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2014•Guangdong nongye kexueOpen access

Study on Stem-tip Tissue Culture and Rapid Propagation Techniques of Strawberry “Ningyu”

Ji Xia

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Abstract

The stolons of strawberry variety Ningyuwere used as explants. After disinfection,we studied and selected the appropriate culture media for stem- tip differentiation,subcultural propagation and rooting. The results showed that the best method of disinfection was using 75% ethanol for 40 s plus 0. 1% HgCl2for 6 ~ 8 min; the best differentiation medium was MS + 0. 5 mg / L 6- BA + 0. 1 mg / L NAA; the best medium for subcultural propagation was MS + 0. 5 mg / L 6- BA + 0. 1 mg / L NAA,and its multiplication coefficient was 9; the best rooting medium was 1 /2MS + 0. 05 mg / L NAA,and the rooting rate reached 96. 6%.

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What this paper is about

The stolons of strawberry variety Ningyuwere used as explants. After disinfection,we studied and selected the appropriate culture media for stem- tip differentiation,subcultural propagation and rooting. The results showed that the best method of disinfection was using 75% ethanol for 40 s plus 0. 1% HgCl2for 6 ~ 8 min; the best differentiation medium was MS + 0. 5 mg / L 6- BA + 0. 1 mg / L NAA; the best medium for subcultural propagation was MS + 0. 5 mg / L 6- BA + 0. 1 mg / L NAA,and its multiplication coefficient was 9; the best rooting medium was 1 /2MS + 0. 05 mg / L NAA,and the rooting rate reached 96. 6%.

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Available abstract

The stolons of strawberry variety Ningyuwere used as explants. After disinfection,we studied and selected the appropriate culture media for stem- tip differentiation,subcultural propagation and rooting. The results showed that the best method of disinfection was using 75% ethanol for 40 s plus 0. 1% HgCl2for 6 ~ 8 min; the best differentiation medium was MS + 0. 5 mg / L 6- BA + 0. 1 mg / L NAA; the best medium for subcultural propagation was MS + 0. 5 mg / L 6- BA + 0. 1 mg / L NAA,and its multiplication coefficient was 9; the best rooting medium was 1 /2MS + 0. 05 mg / L NAA,and the rooting rate reached 96. 6%.

Key concepts: Stolon, Tissue culture, Explant culture, Botany, Horticulture, Plant propagation, Chemistry, Biology

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