Study on Heat Treatment and Shoot Tip Virus Elimination Technique of Strawberry

Taking 'Hongyan'and 'Zhangji' strawberry as material,virus-free materials of strawberry were obtained from meristem culture in vitro,in order to establish the regenerative system by means of plant tissue culture techniques.The results showed that the suitable medium for different culture periods was the suitable medium for differentiation medium was MS+6 BA 0.5 mg/L+NAA 0.02 mg/L;During the proliferating period of clustered shoots,the medium with MS+6 BA 0.5 mg/L+NAA 0.05 mg/L showed the best effects;MS+6 BA 0.2 mg/L+NAA 0.01 mg/L was suitable for rooting and getting strong plants;and the best medium for rooting was a modified 1/2MS+IBA 0.1 mg/L,rooting trte for 100%.The rooted plantlets are transferred into the substrate pearlite∶vermiculite =1∶1,and the survival rate of transplants reached above 95% with the best treatment.