2012Northern HorticultureRequires access

Study on Tissue Culture and Rapid Propagation of 'Dongfangmi No.1' Melon

Li Ren

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Abstract

New borning leaves of 'Dongfangmi No.1' melon were selected as explant and planted into the MS medium with different concentration and variety of endocrine to induce the formation of callus and adventitious buds and roots.The results showed that the most ideal medium for callus inducing was MS+2,4-D 0.10 mg/L+6-BA 1.50 mg/L with the best induction rate 91%;The most ideal medium for adventitious buds inducing was MS+2,4-D 0.01 mg/L+6-BA 1.50 mg/L with the best average 3.52;the best rooting medium was MS+2,4-D 0.05 mg/L+NAA 0.20 mg/L+IBA 0.60 mg/L with the best rooting rate 85%.

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What this paper is about

New borning leaves of 'Dongfangmi No.1' melon were selected as explant and planted into the MS medium with different concentration and variety of endocrine to induce the formation of callus and adventitious buds and roots.The results showed that the most ideal medium for callus inducing was MS+2,4-D 0.10 mg/L+6-BA 1.50 mg/L with the best induction rate 91%;The most ideal medium for adventitious buds inducing was MS+2,4-D 0.01 mg/L+6-BA 1.50 mg/L with the best average 3.52;the best rooting medium was MS+2,4-D 0.05 mg/L+NAA 0.20 mg/L+IBA 0.60 mg/L with the best rooting rate 85%.

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Available abstract

New borning leaves of 'Dongfangmi No.1' melon were selected as explant and planted into the MS medium with different concentration and variety of endocrine to induce the formation of callus and adventitious buds and roots.The results showed that the most ideal medium for callus inducing was MS+2,4-D 0.10 mg/L+6-BA 1.50 mg/L with the best induction rate 91%;The most ideal medium for adventitious buds inducing was MS+2,4-D 0.01 mg/L+6-BA 1.50 mg/L with the best average 3.52;the best rooting medium was MS+2,4-D 0.05 mg/L+NAA 0.20 mg/L+IBA 0.60 mg/L with the best rooting rate 85%.

Key concepts: Melon, Explant culture, Callus, Tissue culture, Botany, Horticulture, Murashige and Skoog medium, Chemistry

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