ScholarLens Back to search
2014•Zhongguo yaofangRequires access

Content Determination of Chlorogenic Acid in Bikang Mixture by HPLC

KE Chang-y

Open publisher page 0 citations

Abstract

OBJECTIVE:To establish a method for the content determination of chlorogenic acid in Bikang mixture.METHODS:HPLC methods was adopted.The determination was performed on Hypersil C18(250 mm×4.6 mm,10 μm)column with mobile phase consisted of 0.5% phosphoric acid-methanol(gradient elution)at the flow rate of 1.0 ml/min.The column temperature was 30 ℃,and the detective wavelength was 327 nm.RESULTS:The linear range of chlorogenic acid were 5.20-62.46 μg/ml(r= 0.999 9)with an average recovery of 98.80%(RSD=0.57%,n=9);RSDs of precision,stability and reproducibility tests were all lower than 2%.CONCLUSIONS:The method is convenient,accurate and reproducible,which can be used for the quality control of Bikang mixture.

About this research paper

What this paper is about

OBJECTIVE:To establish a method for the content determination of chlorogenic acid in Bikang mixture.METHODS:HPLC methods was adopted.The determination was performed on Hypersil C18(250 mm×4.6 mm,10 μm)column with mobile phase consisted of 0.5% phosphoric acid-methanol(gradient elution)at the flow rate of 1.0 ml/min.The column temperature was 30 ℃,and the detective wavelength was 327 nm.RESULTS:The linear range of chlorogenic acid were 5.20-62.46 μg/ml(r= 0.999 9)with an average recovery of 98.80%(RSD=0.57%,n=9);RSDs of precision,stability and reproducibility tests were all lower than 2%.CONCLUSIONS:The method is convenient,accurate and reproducible,which can be used for the quality control of Bikang mixture.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE:To establish a method for the content determination of chlorogenic acid in Bikang mixture.METHODS:HPLC methods was adopted.The determination was performed on Hypersil C18(250 mm×4.6 mm,10 μm)column with mobile phase consisted of 0.5% phosphoric acid-methanol(gradient elution)at the flow rate of 1.0 ml/min.The column temperature was 30 ℃,and the detective wavelength was 327 nm.RESULTS:The linear range of chlorogenic acid were 5.20-62.46 μg/ml(r= 0.999 9)with an average recovery of 98.80%(RSD=0.57%,n=9);RSDs of precision,stability and reproducibility tests were all lower than 2%.CONCLUSIONS:The method is convenient,accurate and reproducible,which can be used for the quality control of Bikang mixture.

Key concepts: Chlorogenic acid, Chromatography, Phosphoric acid, Chemistry, Reproducibility, High-performance liquid chromatography, Content determination, Gradient elution

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Content Determination of Chlorogenic Acid in Bikang Mixture by HPLC — Research Paper | ScholarLens