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2010•Zhongguo yaofangRequires access

Content Determination of Chlorogenic Acid in Rheumatoid II Mixture by HPLC

Jiancheng Zhu

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Abstract

OBJECTIVE: To establish HPLC method for the content determination of chlorogenic acid in Rheumatoid Ⅱ mixture. METHODS: The determination was performed on Diamonsil C18(200 mm×4.6 mm,5 μm) column with mobile phase consisted of acetonitrile-0.4% phosphoric acid (10 ∶ 90) and detection wavelength of 327 nm. RESULTS: The linear range of chlorogenic acid was 24.2～193.6 μg·mL-1(r=0.999 9) with an average recovery of 99.23% (RSD=1.05%,n=9). CONCLUSION: The method is accurate, sensitive and reproducible for the quality control of Rheumatoid Ⅱ mixture.

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What this paper is about

OBJECTIVE: To establish HPLC method for the content determination of chlorogenic acid in Rheumatoid Ⅱ mixture. METHODS: The determination was performed on Diamonsil C18(200 mm×4.6 mm,5 μm) column with mobile phase consisted of acetonitrile-0.4% phosphoric acid (10 ∶ 90) and detection wavelength of 327 nm. RESULTS: The linear range of chlorogenic acid was 24.2～193.6 μg·mL-1(r=0.999 9) with an average recovery of 99.23% (RSD=1.05%,n=9). CONCLUSION: The method is accurate, sensitive and reproducible for the quality control of Rheumatoid Ⅱ mixture.

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Available abstract

OBJECTIVE: To establish HPLC method for the content determination of chlorogenic acid in Rheumatoid Ⅱ mixture. METHODS: The determination was performed on Diamonsil C18(200 mm×4.6 mm,5 μm) column with mobile phase consisted of acetonitrile-0.4% phosphoric acid (10 ∶ 90) and detection wavelength of 327 nm. RESULTS: The linear range of chlorogenic acid was 24.2～193.6 μg·mL-1(r=0.999 9) with an average recovery of 99.23% (RSD=1.05%,n=9). CONCLUSION: The method is accurate, sensitive and reproducible for the quality control of Rheumatoid Ⅱ mixture.

Key concepts: Chlorogenic acid, Phosphoric acid, Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Content determination, Linear relationship

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