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2012•Northwest Pharmaceutical JournalRequires access

Determination of the content of garnenoside in Ankun Tablets by HPLC

Yuanzhi Song

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Abstract

Objective To establish the determination method of gardenoside in Ankun Tablets.Method The quantitative analysis of gardenoside in Ankun Tablets was carried out by HPLC.The chromatographic column was C18(250 mm×4.6 mm,5 μm).The detection wavelength was 238 nm.The mobile phase was acetonitrile-water(15∶85) at a flow rate of 1.0 mL·min-1.The column temperature was 25 ℃.The sample loaded amount was 10 μL.Result The gardenoside appeared linear at the range of 0.122-1.098 μg with correlation coefficient 0.999 5.The average recovery rate was 99.5% with RSD=1.4%(n=6).Conclusion The method is accurate,simple,reproducible,and suitable for the quality control of the preparation.

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What this paper is about

Objective To establish the determination method of gardenoside in Ankun Tablets.Method The quantitative analysis of gardenoside in Ankun Tablets was carried out by HPLC.The chromatographic column was C18(250 mm×4.6 mm,5 μm).The detection wavelength was 238 nm.The mobile phase was acetonitrile-water(15∶85) at a flow rate of 1.0 mL·min-1.The column temperature was 25 ℃.The sample loaded amount was 10 μL.Result The gardenoside appeared linear at the range of 0.122-1.098 μg with correlation coefficient 0.999 5.The average recovery rate was 99.5% with RSD=1.4%(n=6).Conclusion The method is accurate,simple,reproducible,and suitable for the quality control of the preparation.

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Available abstract

Objective To establish the determination method of gardenoside in Ankun Tablets.Method The quantitative analysis of gardenoside in Ankun Tablets was carried out by HPLC.The chromatographic column was C18(250 mm×4.6 mm,5 μm).The detection wavelength was 238 nm.The mobile phase was acetonitrile-water(15∶85) at a flow rate of 1.0 mL·min-1.The column temperature was 25 ℃.The sample loaded amount was 10 μL.Result The gardenoside appeared linear at the range of 0.122-1.098 μg with correlation coefficient 0.999 5.The average recovery rate was 99.5% with RSD=1.4%(n=6).Conclusion The method is accurate,simple,reproducible,and suitable for the quality control of the preparation.

Key concepts: Chromatography, High-performance liquid chromatography, Correlation coefficient, Content determination, Linear correlation, Chemistry, Volumetric flow rate, Analytical Chemistry (journal)

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