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2010•Unpublished venueRequires access

Determination of hesperidins in Jianpizengfei tablets by HPLC

Zhigang Liu

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Abstract

Objective To establish a determination method for hesperidins in Jianpizengfei tablets by HPLC. Methods HPLC was performed on a Kromasil C18 column(250 mm×4.6 mm,5 μm) with Methanol-acetic acid-Water(35∶4∶61) as the mobile phase at a flow rate of 1 mL·min-1 and the detection wavelength was at 283 nm.Results It showed a good linearity at the range of 0.300 8～2.105 6 μg(r=0.999 9,n=7) and the average recovery of hesperidins was 101.68%(RSD=1.45%).Conclusion The method is sensitive,accurate,reproducible,specific and can be used for the quality control of Jianpizengfei tablets efficiently.

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What this paper is about

Objective To establish a determination method for hesperidins in Jianpizengfei tablets by HPLC. Methods HPLC was performed on a Kromasil C18 column(250 mm×4.6 mm,5 μm) with Methanol-acetic acid-Water(35∶4∶61) as the mobile phase at a flow rate of 1 mL·min-1 and the detection wavelength was at 283 nm.Results It showed a good linearity at the range of 0.300 8～2.105 6 μg(r=0.999 9,n=7) and the average recovery of hesperidins was 101.68%(RSD=1.45%).Conclusion The method is sensitive,accurate,reproducible,specific and can be used for the quality control of Jianpizengfei tablets efficiently.

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Available abstract

Objective To establish a determination method for hesperidins in Jianpizengfei tablets by HPLC. Methods HPLC was performed on a Kromasil C18 column(250 mm×4.6 mm,5 μm) with Methanol-acetic acid-Water(35∶4∶61) as the mobile phase at a flow rate of 1 mL·min-1 and the detection wavelength was at 283 nm.Results It showed a good linearity at the range of 0.300 8～2.105 6 μg(r=0.999 9,n=7) and the average recovery of hesperidins was 101.68%(RSD=1.45%).Conclusion The method is sensitive,accurate,reproducible,specific and can be used for the quality control of Jianpizengfei tablets efficiently.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Acetic acid, Biochemistry

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