2010•Zhongguo shiyan fangjixue zazhiRequires access

Determination of Gardenoside in Fushenning Tablets by HPLC

Wang Zhao-hua

Abstract

Objective: To determine the content of Gardenoside in Fushenning Tablets by high performance liquid chromatography.Method: The determination was carried out on a Shim-pack-C18 column(4.6 mm×150 mm,5 μm)eluted with and the mobile phase of acetonitrile-water(10∶90).The detection wavelength was of 238 nm.Result: Gardenoside was linear within the range of 0.136-0.680 μg,with a correlation coefficient of 0.999 9.The average recovery of gardenoside was 98.04%,with RSD 0.46%.Conclusion: The method is simple,accurate,sensitive and can be applied to the quality control of the preparation.

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Key concepts: Chromatography, High-performance liquid chromatography, Gradient elution, Materials science, Correlation coefficient, Content determination, Analytical Chemistry (journal), Chemistry