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Advanced Technique for Silver Staining Methods on Polyacrylamide Gels Electrophoresis

Liao He-rong

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Abstract

To study and modify the optimal methods of silver staining of DNA polyacrylamide gels electrophoresis,polymerase chain reaction(PCR)-polyacrylamide gel electrophoresis silver nitrate(AgNO3)staining were used to detect DNA fragments and pBR322 MspⅠDNA molecular weight.2 silver staining methods were analyzed and evaluated.The sensitivity of all the band of pBR322 MspⅠ Marker developed was coequally 25 ng.However,comparison of time and step,advanced technique was more convenient.The advanced staining cost only about 25 minutes,and was characterized as simple steps,high sensitivity,little pollution,which was recycled and valuable for practical apply.

About this research paper

What this paper is about

To study and modify the optimal methods of silver staining of DNA polyacrylamide gels electrophoresis,polymerase chain reaction(PCR)-polyacrylamide gel electrophoresis silver nitrate(AgNO3)staining were used to detect DNA fragments and pBR322 MspⅠDNA molecular weight.2 silver staining methods were analyzed and evaluated.The sensitivity of all the band of pBR322 MspⅠ Marker developed was coequally 25 ng.However,comparison of time and step,advanced technique was more convenient.The advanced staining cost only about 25 minutes,and was characterized as simple steps,high sensitivity,little pollution,which was recycled and valuable for practical apply.

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Available abstract

To study and modify the optimal methods of silver staining of DNA polyacrylamide gels electrophoresis,polymerase chain reaction(PCR)-polyacrylamide gel electrophoresis silver nitrate(AgNO3)staining were used to detect DNA fragments and pBR322 MspⅠDNA molecular weight.2 silver staining methods were analyzed and evaluated.The sensitivity of all the band of pBR322 MspⅠ Marker developed was coequally 25 ng.However,comparison of time and step,advanced technique was more convenient.The advanced staining cost only about 25 minutes,and was characterized as simple steps,high sensitivity,little pollution,which was recycled and valuable for practical apply.

Key concepts: Silver stain, Staining, Polyacrylamide, Polyacrylamide gel electrophoresis, Gel electrophoresis, Silver nitrate, Gel electrophoresis of nucleic acids, Electrophoresis

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