ScholarLens Back to search
2009•Shandong Agricultural SciencesRequires access

Study on Polyacrylamide Gel Electrophoresis and Silver Staining Methods for Microsatellite DNA

Wenfeng Wang

Open publisher page 0 citations

Abstract

In the study,the polymerase chain reaction(PCR)-polyacrylamide gel electophoresis and silver nitrate staining were used to detect DNA fragments.The results showed that using this method to detect DNA fragments was characterized as high sensitivity,slight stained background and clear bands.So using silver staining to analyze the polyacrylamide gel bands contributed to a longterm preservation and could reduce the pollution to human beings and environment.

About this research paper

What this paper is about

In the study,the polymerase chain reaction(PCR)-polyacrylamide gel electophoresis and silver nitrate staining were used to detect DNA fragments.The results showed that using this method to detect DNA fragments was characterized as high sensitivity,slight stained background and clear bands.So using silver staining to analyze the polyacrylamide gel bands contributed to a longterm preservation and could reduce the pollution to human beings and environment.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

In the study,the polymerase chain reaction(PCR)-polyacrylamide gel electophoresis and silver nitrate staining were used to detect DNA fragments.The results showed that using this method to detect DNA fragments was characterized as high sensitivity,slight stained background and clear bands.So using silver staining to analyze the polyacrylamide gel bands contributed to a longterm preservation and could reduce the pollution to human beings and environment.

Key concepts: Silver stain, Polyacrylamide gel electrophoresis, Polyacrylamide, Staining, Microsatellite, Silver nitrate, Gel electrophoresis, DNA

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Study on Polyacrylamide Gel Electrophoresis and Silver Staining Methods for Microsatellite DNA — Research Paper | ScholarLens