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2004•Zhongguo xin yao zazhiRequires access

HPLC determination of paeoniflorinin in Tianquantong granules

Yonglin Wang

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Abstract

Objective:To establish a HPLC method for determination of Paeoniflorinin in Tianquantong granules.Methods:This system consisted of Hypersil C18 column(150mm×4.6mm,5μm)and methanol-water(30∶70)was used as mobile phase,with the detection wavelength at 230 nm.Results:There was a good linear correlation with paeoniflorinin between 0.201～3.22μg.The average recovery rate of paeoniflorinin was 99.1% and RSD was 2.0%(n=9).Conclusion:The method is simple,accurate,reproducible and can be used for the quality control of paeoniflorinin in Tianquantong granules.

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What this paper is about

Objective:To establish a HPLC method for determination of Paeoniflorinin in Tianquantong granules.Methods:This system consisted of Hypersil C18 column(150mm×4.6mm,5μm)and methanol-water(30∶70)was used as mobile phase,with the detection wavelength at 230 nm.Results:There was a good linear correlation with paeoniflorinin between 0.201～3.22μg.The average recovery rate of paeoniflorinin was 99.1% and RSD was 2.0%(n=9).Conclusion:The method is simple,accurate,reproducible and can be used for the quality control of paeoniflorinin in Tianquantong granules.

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Available abstract

Objective:To establish a HPLC method for determination of Paeoniflorinin in Tianquantong granules.Methods:This system consisted of Hypersil C18 column(150mm×4.6mm,5μm)and methanol-water(30∶70)was used as mobile phase,with the detection wavelength at 230 nm.Results:There was a good linear correlation with paeoniflorinin between 0.201～3.22μg.The average recovery rate of paeoniflorinin was 99.1% and RSD was 2.0%(n=9).Conclusion:The method is simple,accurate,reproducible and can be used for the quality control of paeoniflorinin in Tianquantong granules.

Key concepts: Chromatography, High-performance liquid chromatography, Linear correlation, Linear relationship, Chemistry, Recovery rate, Mathematics, Statistics

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