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Content Determination of Pulchinenoside B_4 in Changfuning Granules by HPLC

Peiyi Liu

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Abstract

OBJECTIVE:To establish a method for the content determination of pulchinenoside B4 in Changfuning granules.METHODS:The determination was performed on the Hypersil C18(250 mm×4.6 mm,5 μm) column at room temperature.The mobile phase was consisted of acetonitrile-0.1%phosphoric acid(30:70) at the flow rate of 1.0 mL·min-1 and detection wavelength of 205 nm.The injection volume was 10 μL.RESULTS:The linear range of pulchinenoside B4 was 3.125～50.000 μg·mL-1(r=0.999 8)with an average recovery of 99.45%(RSD=2.83%,n=6).CONCLUSION:The method is specific,sensitive and reproducibility for the quality control of Changfuning granules.

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OBJECTIVE:To establish a method for the content determination of pulchinenoside B4 in Changfuning granules.METHODS:The determination was performed on the Hypersil C18(250 mm×4.6 mm,5 μm) column at room temperature.The mobile phase was consisted of acetonitrile-0.1%phosphoric acid(30:70) at the flow rate of 1.0 mL·min-1 and detection wavelength of 205 nm.The injection volume was 10 μL.RESULTS:The linear range of pulchinenoside B4 was 3.125～50.000 μg·mL-1(r=0.999 8)with an average recovery of 99.45%(RSD=2.83%,n=6).CONCLUSION:The method is specific,sensitive and reproducibility for the quality control of Changfuning granules.

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Available abstract

OBJECTIVE:To establish a method for the content determination of pulchinenoside B4 in Changfuning granules.METHODS:The determination was performed on the Hypersil C18(250 mm×4.6 mm,5 μm) column at room temperature.The mobile phase was consisted of acetonitrile-0.1%phosphoric acid(30:70) at the flow rate of 1.0 mL·min-1 and detection wavelength of 205 nm.The injection volume was 10 μL.RESULTS:The linear range of pulchinenoside B4 was 3.125～50.000 μg·mL-1(r=0.999 8)with an average recovery of 99.45%(RSD=2.83%,n=6).CONCLUSION:The method is specific,sensitive and reproducibility for the quality control of Changfuning granules.

Key concepts: Chromatography, Phosphoric acid, Reproducibility, Content determination, Chemistry, Linear range, High-performance liquid chromatography, Linear relationship

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Content Determination of Pulchinenoside B_4 in Changfuning Granules by HPLC — Research Paper | ScholarLens