Resveratrol Induces Apoptosis of MDA-MB-231 Cells and Increases the Reactive Oxygen Species and Expression of Caspase-3 of the Cells

Objective To investigate the apoptosis of MDA-MB-231 cells induced by resveratrol and the influence resveratrol on the intracellular reactive oxygen species(ROS)level and Caspase-3.Methods MDA-MB-231 cells were treated with resveratrol in different concentrations for 24 h.Flow cytometry(FCM)and DNA agarose gel electrophoresis were used to detect the apoptosis of MDA-MB-231 cells.Intracellular ROS level was measured with FCM,and the expression of Caspase-3 was determined by immunohistochemistry.Results Resveratrol could induce the apoptosis of MDA-MB-231 cells.Agarose gel electrophoresis showed characteristic DNA ladder fragmentation,as well as the typical change of apoptosis.The level of ROS in MDA-MB-231 cells were(50.0±5.7),(115.0±10.6)and(79.2±6.9),respectively,after treatment by 60 μmol/L resveratrol for 4h,12h and 24h.Caspase-3 was also activated by Resveratrol.Conclusion Resveratrol induced apoptosis is related to the increased ROS and activated Caspase-3 in MDA-MB-231 cells.It is may be one of the mechanisms of apoptosis induced by Resveratrol in MDA-MB-231 cells.