Effects of resveratrol on cell morphology,mitochondrial membrane potential,reactive oxygen species,and intracellular calcium concentration in SGC-7901

Objective To investigate the effect of resveratrol on human gastric cancer SGC-7901 cell line.Methods SGC-7901 cell line was cultured in vitro for 48 h.The end concentration of resveratrol low-,mid-,and high-dose groups was 44,88,and 176 μmol/L,respectively;The end concentration of positive control group(5-FU) was 153.8 μmol/L;Negative control group was administrated with the same volume of culture medium without drugs.Morphology of tumor cells treated with different concentration of resveratrol was identified by fluorescence microscope.Flow cytometry was used to detect the influence of resveratrol on the changes of mitochondrial membrane potential(MMP) and reactive oxygen species(ROS) in tumor cells.After the tumor cell was exposed to resveratrol,the intracellular calcium concentration([Ca2+]i) was measured with laser scanning confocal microscope.Results According to the morphology,the tumor cell staining was deepened;Chromatin aggregation and fragmentation were observed,and the apoptosis bodies were generated.With increasing the concentration of resveratrol,the phenomenon was more obvious,which indicated that the proportion of apoptosis increased.Resveratrol could reduce MMP of tumor cells,and ROS of tumor cells has also increased with increasing concentration of resveratrol.It indicated that resveratrol could induce tumor cell apoptosis by affecting the content of ROS.The high doses could improve [Ca2+]i significantly in a dose-dependent manner.Conclusion Resveratrol could induce tumor cell apoptosis by affecting MMP,ROS,and [Ca2+]i.