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Changes of reactive oxygen species in apoptosis induced by resveratrol in K562 cells

Hao Chun

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Abstract

AIM:To investigate the apoptosis of K562 cells induced by resveratrol and the influence of intracellular reactive oxygen species (ROS) level. METHODS: MTT assay, light microscopy, DNA agarose gel electrophoresis and cell cycle analysis were used for examine apoptosis in K562 cells. Intracellular ROS level was measured with flow cytometry (FCM). RESULTS: Resveratrol inhibited growth of K562 cells. Agarose gel electrophoresis showed evident DNA fragmentation. Cell cycle analysis indicated increased S phase proportion, as well as apparent S/G 2 phase arrest. After application of resveratrol, the level of the markedly increased of ROS. CONCLUSION: Resveratrol induces apoptosis in K562 cells may be through stimulating ROS production.

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What this paper is about

AIM:To investigate the apoptosis of K562 cells induced by resveratrol and the influence of intracellular reactive oxygen species (ROS) level. METHODS: MTT assay, light microscopy, DNA agarose gel electrophoresis and cell cycle analysis were used for examine apoptosis in K562 cells. Intracellular ROS level was measured with flow cytometry (FCM). RESULTS: Resveratrol inhibited growth of K562 cells. Agarose gel electrophoresis showed evident DNA fragmentation. Cell cycle analysis indicated increased S phase proportion, as well as apparent S/G 2 phase arrest. After application of resveratrol, the level of the markedly increased of ROS. CONCLUSION: Resveratrol induces apoptosis in K562 cells may be through stimulating ROS production.

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Available abstract

AIM:To investigate the apoptosis of K562 cells induced by resveratrol and the influence of intracellular reactive oxygen species (ROS) level. METHODS: MTT assay, light microscopy, DNA agarose gel electrophoresis and cell cycle analysis were used for examine apoptosis in K562 cells. Intracellular ROS level was measured with flow cytometry (FCM). RESULTS: Resveratrol inhibited growth of K562 cells. Agarose gel electrophoresis showed evident DNA fragmentation. Cell cycle analysis indicated increased S phase proportion, as well as apparent S/G 2 phase arrest. After application of resveratrol, the level of the markedly increased of ROS. CONCLUSION: Resveratrol induces apoptosis in K562 cells may be through stimulating ROS production.

Key concepts: Agarose gel electrophoresis, Resveratrol, Reactive oxygen species, Apoptosis, DNA fragmentation, Flow cytometry, Intracellular, Cell cycle

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