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2001•Carcinogenesis,Teratogenesis and MutagenesisRequires access

INFLUENCE ON THE TUMORIGENICITY OF THE HUMAN RENAL CELL CARCINOMA (RCC) CELLS TRANSDUCED WITH HUMAN TNFα GENE

Xiao Zhang

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Abstract

Purpose: To study the influence on the tumorigenicity of the human renal cell carcinoma (RCC) cells by transfection of human TNFα gene. Methods: hTNFα cDNA was inserted into retroviral vector to construct recombinant vector pL(TNFα)SN. The packaged and confirmed retrovirus were infected to RCC cell line. Then, nude mice were injected by 786 0 cell infected with the hTNFα gene or not , and the tumorigenecity of RCC was observed. Results: The mean expression level of TNFα in the supernatant of TNFα transfected 786 0 cell culture medium was (5 004±624) pg/ml. And the nude mice injected with TNFα gene engineered RCC cells had no tumor growth. Conclusion: The TNFα gene engineered human RCC cells lost tumorigenicity.

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What this paper is about

Purpose: To study the influence on the tumorigenicity of the human renal cell carcinoma (RCC) cells by transfection of human TNFα gene. Methods: hTNFα cDNA was inserted into retroviral vector to construct recombinant vector pL(TNFα)SN. The packaged and confirmed retrovirus were infected to RCC cell line. Then, nude mice were injected by 786 0 cell infected with the hTNFα gene or not , and the tumorigenecity of RCC was observed. Results: The mean expression level of TNFα in the supernatant of TNFα transfected 786 0 cell culture medium was (5 004±624) pg/ml. And the nude mice injected with TNFα gene engineered RCC cells had no tumor growth. Conclusion: The TNFα gene engineered human RCC cells lost tumorigenicity.

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Available abstract

Purpose: To study the influence on the tumorigenicity of the human renal cell carcinoma (RCC) cells by transfection of human TNFα gene. Methods: hTNFα cDNA was inserted into retroviral vector to construct recombinant vector pL(TNFα)SN. The packaged and confirmed retrovirus were infected to RCC cell line. Then, nude mice were injected by 786 0 cell infected with the hTNFα gene or not , and the tumorigenecity of RCC was observed. Results: The mean expression level of TNFα in the supernatant of TNFα transfected 786 0 cell culture medium was (5 004±624) pg/ml. And the nude mice injected with TNFα gene engineered RCC cells had no tumor growth. Conclusion: The TNFα gene engineered human RCC cells lost tumorigenicity.

Key concepts: Transfection, Renal cell carcinoma, Cell culture, Tumor necrosis factor alpha, Viral vector, Retrovirus, Cancer research, Recombinant DNA

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INFLUENCE ON THE TUMORIGENICITY OF THE HUMAN RENAL CELL CARCINOMA (RCC) CELLS TRANSDUCED WITH HUMAN TNFα GENE — Research Paper | ScholarLens