Loss of Tumorigenicity of the Primary Renal Cell Carcinoma by Transduced with Human IL-2 Gene

Objective To study the tumorigenicity of the human renal cell carcinoma(RCC)cells transduced with human IL-2 gene.Methods Recombinant retroviral vector pLNC(IL-2) was constructed and transfected into the packaged cell.The primary RCC cells were infected with confirmed retrovirus and the biological activity of rhIL-2 was tested by MTT method.Then,primary RCC cell,infected with the hIL-2 gene or not,was injected into nude mice and the tumorigenecity of RCC was observed.Results The biological activity of rhIL-2 in the recominbinant retrovirus supernants was meanly(244±52) U/ml.The nude mice injected with IL-2 gene engineered RCC cells had no tumor growth.Conclusion The IL-2 gene engineered human RCC cells lost tumorigenicity.The primary RCC cells transduced with human IL-2 gene might be as tumor vaccine for the treatment of RCC.