SUPPRESSION OF TUMORIGENICITY OF PRIMARY RENAL CELL CARCINOMA (RCC) CELLS TRANSFECTED WITH HUMAN IL -2 GENE

Objective To study the influence on tumorigenicity of human renal cell carcinoma (RCC) cells transduced with human IL 2 gene, and to intend in forming a basis for RCC gene therapy.Methods Recombinant retroviral vector pLNC (IL 2) was constructed and transfected into the packaged cell. Primary RCC cells were infected with confirmed retrovirus, and the biological activity of rhIL 2 was tested by MTT method. Then, nude mice were injected with primary RCC cell infected with or without the hIL 2 gene, and the tumorigenecity of RCC was observed.Results The biological activity of rhIL 2 in the recombinant retrovirus supernatants was 244±52 U/ml. The nude mice injected with IL 2 gene enginerred RCC cells had no tumor growth. Conclusion The IL 2 gene engineered human RCC cells lost tumorigenicity. The primary RCC cells transduced with human IL 2 gene might be used as tumor vaccine to treat the RCC.$$$$