2007•Central South PharmacyRequires access

Determination of icariin in Yishen Kanggu tablets by RP-HPLC

Huang Ke

Abstract

Objective To establish a method for the determination of icariin in Yishen Kanggu tablets by RP-HPLC.Methods RP-HPLC method was used.The samples were separated on Shim-pack HPLC column with the mobile phase of methanol-1% glacial acetic acid(60∶40).The flow-rate was 1.0 mL·min-1 at 30 ℃.The detection wavelength was 270 nm.Results The linear calibration curves were obtained over 0.1344～1.344 μg(r=0.9997).The average recovery of icariin was 98.76%(RSD=1.69%,n=5) in the tablets.The method was precise(RSD= 0.87%).Conclusion The method is quick,accurate and suitable for detecting the content of icariin in Yishen Kanggu tablets.

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Available abstract

Key concepts: Icariin, Chromatography, High-performance liquid chromatography, Chemistry, Content determination, Calibration curve, Acetic acid, Detection limit