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2005•Chinese New Drugs JournalRequires access

Quantification of icariin in tonifying kidney and vital essence tablets

Cui Xiao-hong

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Abstract

Objective:To quantify icariin from Herb Epimedh in tonifying kidney and vital essence (Yishenqiangshen) tablets.Methods:Icariin in the tables was quantified by Reversed Phase-High Performance Liquid Chromatography composed of a Diamonsil C_(18) column(250 mm×4.6 mm,5 μm),an eluate of(CH_3OH-)H_2O(60∶40) with a flow rate of 1 ml·min~(-1),and a detection wavelength at 268 nm.Results:The linearity of icariin concentration curve was in the range of 0.201～4.020 μg·L~(-1)(r=0.999).The average recovery of icariin was 97.92% and a RSD was 0.55%.Conclusion:This reproducible and accurate HPLC method can be used for quality control of the Yishenqiangshen tablets.

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Objective:To quantify icariin from Herb Epimedh in tonifying kidney and vital essence (Yishenqiangshen) tablets.Methods:Icariin in the tables was quantified by Reversed Phase-High Performance Liquid Chromatography composed of a Diamonsil C_(18) column(250 mm×4.6 mm,5 μm),an eluate of(CH_3OH-)H_2O(60∶40) with a flow rate of 1 ml·min~(-1),and a detection wavelength at 268 nm.Results:The linearity of icariin concentration curve was in the range of 0.201～4.020 μg·L~(-1)(r=0.999).The average recovery of icariin was 97.92% and a RSD was 0.55%.Conclusion:This reproducible and accurate HPLC method can be used for quality control of the Yishenqiangshen tablets.

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Available abstract

Objective:To quantify icariin from Herb Epimedh in tonifying kidney and vital essence (Yishenqiangshen) tablets.Methods:Icariin in the tables was quantified by Reversed Phase-High Performance Liquid Chromatography composed of a Diamonsil C_(18) column(250 mm×4.6 mm,5 μm),an eluate of(CH_3OH-)H_2O(60∶40) with a flow rate of 1 ml·min~(-1),and a detection wavelength at 268 nm.Results:The linearity of icariin concentration curve was in the range of 0.201～4.020 μg·L~(-1)(r=0.999).The average recovery of icariin was 97.92% and a RSD was 0.55%.Conclusion:This reproducible and accurate HPLC method can be used for quality control of the Yishenqiangshen tablets.

Key concepts: Icariin, Chromatography, High-performance liquid chromatography, Chemistry, Elution, Medicine, Pathology, Alternative medicine

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