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2006•Zhongguo yaofangRequires access

Determination of Icariin in Kangguzengsheng Pills by HPLC

Lifang Yang

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Abstract

OBJECTIVE:To determine the content of Icariin in Kangguzengsheng pills by HPLC.METHODS:The separation was performed on Diamansil C 18 ;the mobile phase was composed of acetonitrile and water(29∶71)with a flow rate of0.8ml/min.The detection wavelength was270nm and the column temperature was35℃.RESULTS:The1inear range of icariin was0.0618μg～0.309μg(r=1.0000);the average recovery was99.55%(RSD=0.8%).CONCLUSION:The method is precise,accurate,and suitable for the quality control of Kangguzengsheng Pills.

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What this paper is about

OBJECTIVE:To determine the content of Icariin in Kangguzengsheng pills by HPLC.METHODS:The separation was performed on Diamansil C 18 ;the mobile phase was composed of acetonitrile and water(29∶71)with a flow rate of0.8ml/min.The detection wavelength was270nm and the column temperature was35℃.RESULTS:The1inear range of icariin was0.0618μg～0.309μg(r=1.0000);the average recovery was99.55%(RSD=0.8%).CONCLUSION:The method is precise,accurate,and suitable for the quality control of Kangguzengsheng Pills.

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Available abstract

OBJECTIVE:To determine the content of Icariin in Kangguzengsheng pills by HPLC.METHODS:The separation was performed on Diamansil C 18 ;the mobile phase was composed of acetonitrile and water(29∶71)with a flow rate of0.8ml/min.The detection wavelength was270nm and the column temperature was35℃.RESULTS:The1inear range of icariin was0.0618μg～0.309μg(r=1.0000);the average recovery was99.55%(RSD=0.8%).CONCLUSION:The method is precise,accurate,and suitable for the quality control of Kangguzengsheng Pills.

Key concepts: Icariin, Chromatography, High-performance liquid chromatography, Chemistry, Content determination, Pill, Chromatographic separation, Linear range

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