Effects of Nourishing Yin and Promoting Blood Flow Recipe on oxidative stress level and pathologic changes in diabetic rat liver

Objective To discuss the effects of Nourishing Yin and Promoting Blood Flow Recipe on oxidative stress level and pathologic changes in diabetic rat liver. Methods Diabetic rats were induced by intraperitoneal streptozotocin(STZ) injection. Successfully-established model rats were selected and randomly divided into four groups: diabetic group, traditional Chinese medicine(TCM) six-week intervention group, TCM eight-week intervention group, TCM tenweek intervention group. In addition, eight normal rats were randomly chosen as normal control group. Rat livers were extracted respectively after six weeks, eight weeks and ten weeks to determine oxidation index and undertake pathologic observation. Results Compared with normal control group, contents of malonaldehyde(MDA) in rat livers of diabetic group rose significantly(P 0.01), activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) were notably lower than those of normal control group(P 0.01); compared with diabetic group, contents of MDA in rat livers of TCM intervention group in each period decreased strikingly(P 0.05 or P 0.01), activities of SOD and GSH-Px in TCM eight-week intervention group and TRC ten-week intervention group increased significantly(P 0.05 or P 0.01).Rising tendency was shown from activities of SOD and GSH-Px in TCM six-week intervention group, but there was no statistics difference(P 0.05). By pathology observation, liver cell cords and hepatic lobules structure of diabetic group were chaotic, with inflammatory cells increasing, which indicated that there were injuries in hepatic tissue. Compared with diabetic group, morphology changes were improved in every phase of TCM intervention group. Conclusion Certain anti-oxidative capability was shown to restrain oxidative stress in Nourishing Yin and Promoting Blood Flow Recipe,which has some protection to diabetic rat liver.