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Determination of Oxymatrine by HPLC

Xiaoshan Wang

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Abstract

To establish a method for determination of oxymatrine with HPLC,a C 18 column and the mobile phase of MeOH-3%H3 PO4 (8∶2,v∶v) were used. The detection wavelength and temperature were set at 220 nm and 30 ℃,and the flow rate was 1.0 mL/min. The calibration curve of oxymatrine content was linear within the range of 1～10 μg (r=0.9998),the average recovery of oxymatrine was 99.33%,and RSD was 0.296%. The HPLC method is accurate and sensitive for determining oxymatrine.

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What this paper is about

To establish a method for determination of oxymatrine with HPLC,a C 18 column and the mobile phase of MeOH-3%H3 PO4 (8∶2,v∶v) were used. The detection wavelength and temperature were set at 220 nm and 30 ℃,and the flow rate was 1.0 mL/min. The calibration curve of oxymatrine content was linear within the range of 1～10 μg (r=0.9998),the average recovery of oxymatrine was 99.33%,and RSD was 0.296%. The HPLC method is accurate and sensitive for determining oxymatrine.

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Available abstract

To establish a method for determination of oxymatrine with HPLC,a C 18 column and the mobile phase of MeOH-3%H3 PO4 (8∶2,v∶v) were used. The detection wavelength and temperature were set at 220 nm and 30 ℃,and the flow rate was 1.0 mL/min. The calibration curve of oxymatrine content was linear within the range of 1～10 μg (r=0.9998),the average recovery of oxymatrine was 99.33%,and RSD was 0.296%. The HPLC method is accurate and sensitive for determining oxymatrine.

Key concepts: Oxymatrine, Chemistry, Chromatography, High-performance liquid chromatography, Calibration curve, Linear range, Analytical Chemistry (journal), Detection limit

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