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2015•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of carbohydrate in Reduning Injection by HPLC- ELSD

BI Yu-a

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Abstract

Objective To establish a method for the determination of fructose and glucose by HPLC- ELSD in Reduning injection. Methods The aminopropyl column was used with acetonitrile: water( 83∶ 17) as mobile phase at a flow rate of 0. 7 m L / min and ELSD detector,the flow rate was 1. 2ml / min. Results The calibration linear curves of fructose and glucose were within concentrations of 0. 4056 ~ 4. 056 g and 0. 8348 ~ 8. 348 g. The average recoveries of fructose and glucose were 102. 3% and 102.0%,RSD were 2. 8% and 2. 9% respectively. Conclusion This method is simple,quick and specific,and suitable for the quality control both of the components in Reduning injection.

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What this paper is about

Objective To establish a method for the determination of fructose and glucose by HPLC- ELSD in Reduning injection. Methods The aminopropyl column was used with acetonitrile: water( 83∶ 17) as mobile phase at a flow rate of 0. 7 m L / min and ELSD detector,the flow rate was 1. 2ml / min. Results The calibration linear curves of fructose and glucose were within concentrations of 0. 4056 ~ 4. 056 g and 0. 8348 ~ 8. 348 g. The average recoveries of fructose and glucose were 102. 3% and 102.0%,RSD were 2. 8% and 2. 9% respectively. Conclusion This method is simple,quick and specific,and suitable for the quality control both of the components in Reduning injection.

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Available abstract

Objective To establish a method for the determination of fructose and glucose by HPLC- ELSD in Reduning injection. Methods The aminopropyl column was used with acetonitrile: water( 83∶ 17) as mobile phase at a flow rate of 0. 7 m L / min and ELSD detector,the flow rate was 1. 2ml / min. Results The calibration linear curves of fructose and glucose were within concentrations of 0. 4056 ~ 4. 056 g and 0. 8348 ~ 8. 348 g. The average recoveries of fructose and glucose were 102. 3% and 102.0%,RSD were 2. 8% and 2. 9% respectively. Conclusion This method is simple,quick and specific,and suitable for the quality control both of the components in Reduning injection.

Key concepts: Fructose, Chromatography, Chromatography detector, High-performance liquid chromatography, Calibration curve, Chemistry, Detection limit, Biochemistry

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