Determination of Four Saccharides in Qingkailing Injection by HPLC-ELSD

OBJECTIVE To establish an HPLC-ELSD method to determine the contents of four saccharides in Qingkailing injection and carry out cluster analysis of the products from different manufactures.METHODS Chromatographic separation was performed on a Prevail Carbohydrate ES column with mobile phase of acetonitrile(B)-water(A) at a flow rate of 1.0 mL·min-1 with gradient elution;the column temperature was 30 ℃;the ELSD drift tube temperature was 90.7 ℃ and the gas flow was 2.4 L·min-1.Cluster analysis was performed by SPSS16.0.RESULTS Good resolution and linearities were obtained.The average recoveries of xylose,fructose,glucose and sucrose were 98.52%(RSD=1.70%),99.15%(RSD=2.55%),99.82%(RSD=2.04%) and 99.26%(RSD=1.67%),respectively.The test samples were clustered into three classes.CONCLUSION The established method is simple,reliable and accurate.It can be used to determine the saccharide components in Qingkailing injection,and provide theoretical basis for its quality control.