Content Determination of Magnolol and Honokiol in Magnolia officinalis in Xiangsha Yangwei Pill

[Objective] The research aimed to establish the determination method of the contents of magnolol and honokiol in Xiangsha Yangwei Pill.[Method] HPLC was used to measure the content of magnolol and honlkiol.Phenomenex Kromasil C18(250 mm 4.6 mm,5 μm) column was used,and mobile phase was composed of acetonitrile-water-glacial acetic acid(55∶43∶2).Detection wavelength was at 294 nm,the flow rate was 1.0 ml/min,the injection volume was 20 μl.[Result] The linear scope of measuring the contents of magnolol and honokiol were 0.190 2-3.804 0 μg and 0.092 222-1.844 400 μg resp..The average recovery ratio was 97.0% and RSD was 1.7%.[Conclusion] The method is accurate,easy to operate,and it can be used for quality control of the product identification to provide basis for the establishment of integrated quality standards.