Determination on Magnolol and Honokiol Contents in Huoxiangzhengqi Tincture by RP-HPLC Method

[Objective] The aim of the study was to determine magnolol and honokiol contents in Huoxiangzhengqi tincture.[Method] With Diamonsil(TM)C18 column(250 mm × 4.6 mm,5 μm),methanol-water-acetic acid(79∶21∶0.25) as mobile phase,flow velocity at 1.0 mL/min,detection wave length at 294 nm and column temperature at 23 ℃,the magnolol and honokiol contents in Huoxiangzhengqi water were determined by RP-HPLC.[Result] The magnolol and honokiol contents determined by RP-HPLC method had good linear relationship with the peak areas when their concentrations were at 6.25～400 μg/mL.The average recoveries and RSDs of magnolol were 100.78 % and 99.6 % resp.and those of honokiol were 1.52 % and 1.18 % resp.[Conclusion] This method was simple and convenient,accurate and specific,which could control the checking quality of Huoxiangzhengqi tincture effectively.