Determination of magnolol and honokiol in XIANGSHA YANGWEI GRANULES by RP-HPLC
Lin Wang
Abstract
Lin Wang
Abstract
Objective:To establish a method to determine the contents of magnolol and honokiol in XIANGSHA YANGWEI GRANULES by RP-HPLC.Method:A ODS column was used.The mobile phase was methanol-water (70∶30).The flow rate was 1.0mL/min and the detective wavelength was 315nm.Results:Linear range of magnolol and honokiol were 0^235~1^410μg( r 0^9998) and 0^358~2^148μg( r 0^9997),respectively.The average recoveries were 99.42%(RSD was 0.66%) and 97.15% (RSD was 1.04%),respectively.Conclusion:The method was accurate and reliable.
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Objective:To establish a method to determine the contents of magnolol and honokiol in XIANGSHA YANGWEI GRANULES by RP-HPLC.Method:A ODS column was used.The mobile phase was methanol-water (70∶30).The flow rate was 1.0mL/min and the detective wavelength was 315nm.Results:Linear range of magnolol and honokiol were 0^235~1^410μg( r 0^9998) and 0^358~2^148μg( r 0^9997),respectively.The average recoveries were 99.42%(RSD was 0.66%) and 97.15% (RSD was 1.04%),respectively.Conclusion:The method was accurate and reliable.
Key concepts: Honokiol, Magnolol, Chromatography, Chemistry, High-performance liquid chromatography, Linear range, Detection limit