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2009•Lishizhen Medicine and Materia Medica ResearchRequires access

Impovement of HPLC Determination of Ginsenoside Re in Ginsenosides

Zhao Guang-cai

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Abstract

Objective To improve the HPLC method for determination of ginsenosid Re in ginsenosides.Methods ODS column(150 mm×4.6 mm,5 μm) was used.The mobile phase consisted of CH3CN-H2O,the detection wavelength was 203 nm,and the flow rate was 1.0 ml/min.Results The regression equation of ginsenoside Re was Y=9 505.448 8X-1 159.607 0 with a correlation coefficient of 0.999 8,and linear range was within the range of 1.88 ~60.16 μg/ml,the average recovery of ginsenosid Re was 99.35%(RSD=0.49%,n=6).Conclusion This method is simple,quick,and reproducible and it is suitable for the quality control of Panax ginseng.

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What this paper is about

Objective To improve the HPLC method for determination of ginsenosid Re in ginsenosides.Methods ODS column(150 mm×4.6 mm,5 μm) was used.The mobile phase consisted of CH3CN-H2O,the detection wavelength was 203 nm,and the flow rate was 1.0 ml/min.Results The regression equation of ginsenoside Re was Y=9 505.448 8X-1 159.607 0 with a correlation coefficient of 0.999 8,and linear range was within the range of 1.88 ~60.16 μg/ml,the average recovery of ginsenosid Re was 99.35%(RSD=0.49%,n=6).Conclusion This method is simple,quick,and reproducible and it is suitable for the quality control of Panax ginseng.

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Available abstract

Objective To improve the HPLC method for determination of ginsenosid Re in ginsenosides.Methods ODS column(150 mm×4.6 mm,5 μm) was used.The mobile phase consisted of CH3CN-H2O,the detection wavelength was 203 nm,and the flow rate was 1.0 ml/min.Results The regression equation of ginsenoside Re was Y=9 505.448 8X-1 159.607 0 with a correlation coefficient of 0.999 8,and linear range was within the range of 1.88 ~60.16 μg/ml,the average recovery of ginsenosid Re was 99.35%(RSD=0.49%,n=6).Conclusion This method is simple,quick,and reproducible and it is suitable for the quality control of Panax ginseng.

Key concepts: Chromatography, Ginseng, Ginsenoside, Correlation coefficient, High-performance liquid chromatography, Chemistry, Linear range, Linear relationship

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