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2012•Anhui Medical and Pharmaceutical JournalRequires access

Determination of Ginsenoside Rg_1 and Re in Gucining Tablets by HPLC

Chen Yong-lan

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Abstract

Objective To establish an HPLC method for determining Ginsenoside Rg1 and Re in Gucining tablets.Methods C18 column(250 mm×4.6 mm,5 μm) was adopted and the mobile phase was composed of acetonitrile-0.05 mol·L-1 phosphoric acid(20∶ 80).The flow rate was 1.0 ml·min-1.Diode array detector was set at 203 nm.Results The linear range of Ginsenoside Rg1 was between 0.127 and 1.524 g·L-1(r=0.999 8,n=6) and the average recovery was 97.8%,RSD being 0.75%(n=6).The linear range of Ginsenoside Re was between 0.023 and 0.276 g·L-1(r=0.999 7,n=6) and the average recovery was 99.9%,RSD being 0.46%(n=6).Conclusion The method is accurate and simple for the quality control of the preparation.

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Objective To establish an HPLC method for determining Ginsenoside Rg1 and Re in Gucining tablets.Methods C18 column(250 mm×4.6 mm,5 μm) was adopted and the mobile phase was composed of acetonitrile-0.05 mol·L-1 phosphoric acid(20∶ 80).The flow rate was 1.0 ml·min-1.Diode array detector was set at 203 nm.Results The linear range of Ginsenoside Rg1 was between 0.127 and 1.524 g·L-1(r=0.999 8,n=6) and the average recovery was 97.8%,RSD being 0.75%(n=6).The linear range of Ginsenoside Re was between 0.023 and 0.276 g·L-1(r=0.999 7,n=6) and the average recovery was 99.9%,RSD being 0.46%(n=6).Conclusion The method is accurate and simple for the quality control of the preparation.

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Available abstract

Objective To establish an HPLC method for determining Ginsenoside Rg1 and Re in Gucining tablets.Methods C18 column(250 mm×4.6 mm,5 μm) was adopted and the mobile phase was composed of acetonitrile-0.05 mol·L-1 phosphoric acid(20∶ 80).The flow rate was 1.0 ml·min-1.Diode array detector was set at 203 nm.Results The linear range of Ginsenoside Rg1 was between 0.127 and 1.524 g·L-1(r=0.999 8,n=6) and the average recovery was 97.8%,RSD being 0.75%(n=6).The linear range of Ginsenoside Re was between 0.023 and 0.276 g·L-1(r=0.999 7,n=6) and the average recovery was 99.9%,RSD being 0.46%(n=6).Conclusion The method is accurate and simple for the quality control of the preparation.

Key concepts: Ginsenoside Rg1, Chromatography, Phosphoric acid, High-performance liquid chromatography, Linear range, Chromatography detector, Ginsenoside, Chemistry

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