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2011•Zhonghua zhongyiyao xuekanRequires access

Determination of the Content Changes of Ginsenoside Rg_3 in Panax Pseudo-ginseng Fermentation Product by HPLC

Qing Wang

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Abstract

Objective: To establish a assay method of ginsenoside Rg3 in Panax pseudo-ginseng before and after the fungal transformation by HPLC.Methods: A Phenomex C18(250mm×4.6mm,5μm) column served as stationary phase and acetonitrile-water in gradient elution mode as mobile phase;The column temperature was 30℃ and the detection wavelength was 203nm,the flow rate was 1.0mL/min and the injection volume was 20μL.Results: A good linearity of ginsenoside Rg3 was in the range of 0.0019~ 0.2488 mg/mL and r=0.99992;The average recovery rate was 99.56% and RSD = 1.48%(n= 8).Conclusion: This method is simple,fast,accurate,reproducible and can be used for determining the content of ginsenoside Rg3 in Panax pseudo-ginseng before and after the fungal transformation.

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What this paper is about

Objective: To establish a assay method of ginsenoside Rg3 in Panax pseudo-ginseng before and after the fungal transformation by HPLC.Methods: A Phenomex C18(250mm×4.6mm,5μm) column served as stationary phase and acetonitrile-water in gradient elution mode as mobile phase;The column temperature was 30℃ and the detection wavelength was 203nm,the flow rate was 1.0mL/min and the injection volume was 20μL.Results: A good linearity of ginsenoside Rg3 was in the range of 0.0019~ 0.2488 mg/mL and r=0.99992;The average recovery rate was 99.56% and RSD = 1.48%(n= 8).Conclusion: This method is simple,fast,accurate,reproducible and can be used for determining the content of ginsenoside Rg3 in Panax pseudo-ginseng before and after the fungal transformation.

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Available abstract

Objective: To establish a assay method of ginsenoside Rg3 in Panax pseudo-ginseng before and after the fungal transformation by HPLC.Methods: A Phenomex C18(250mm×4.6mm,5μm) column served as stationary phase and acetonitrile-water in gradient elution mode as mobile phase;The column temperature was 30℃ and the detection wavelength was 203nm,the flow rate was 1.0mL/min and the injection volume was 20μL.Results: A good linearity of ginsenoside Rg3 was in the range of 0.0019~ 0.2488 mg/mL and r=0.99992;The average recovery rate was 99.56% and RSD = 1.48%(n= 8).Conclusion: This method is simple,fast,accurate,reproducible and can be used for determining the content of ginsenoside Rg3 in Panax pseudo-ginseng before and after the fungal transformation.

Key concepts: Ginseng, Chromatography, Ginsenoside, Chemistry, High-performance liquid chromatography, Gradient elution, Content determination, Traditional medicine

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Determination of the Content Changes of Ginsenoside Rg_3 in Panax Pseudo-ginseng Fermentation Product by HPLC — Research Paper | ScholarLens